Biochem/physiol Actions

Target IC₅₀: 23 nM, 4 nM and 18 nM for ALK5 binding, ALK5 auto-phosphorylation, and TGF-&beta

Reversible: yes

Cell permeable: yes

Primary TargetALK5

General description

A cell-permeable, potent, selective, reversible, and ATP-competitive inhibitor of TGF-β type I receptor (ALK5; IC₅₀ = 23 nM, 4 nM and 18 nM for ALK5 binding, ALK5 auto-phosphorylation, and TGF-β cellular assay in HepG2 cells, respectively). Minimally affects a panel of 9 closely related kinases including p38 MAPK (IC₅₀ >16 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ichida, J. K., et al. 2009. Cell Stem Cell5, 491.Gellibert, F., et al. 2004. J. Med. Chem.47, 4494.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Physical form

A 50 mM (5 mg/348 µl) sterile-filtered solution of TGF-β RI Kinase Inhibitor II, RepSox (Cat. No. 616452) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. Use only fresh DMSO for further dilution.

Warning

Toxicity: Standard Handling (A)